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相关概念视频

MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

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Experimental RNAi02:15

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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相关实验视频

Updated: Jun 29, 2025

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

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用小分子准微RNA.

Kisanet Tadesse1, Raphael I Benhamou1

  • 1The Institute for Drug Research of the School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 91120, Israel.

Non-coding RNA
|March 25, 2024
PubMed
概括
此摘要是机器生成的。

小分子正在成为精确向涉及疾病的微RNA (miRs) 的强大工具. 这种方法通过调节miR活动,为各种疾病提供了新的治疗策略.

关键词:
里博塔克 (RIBOTAC) 是一个这是一个微型RNA.小分子的小分子.有针对性的降解降解目标.治疗药物 治疗药物

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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
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相关实验视频

Last Updated: Jun 29, 2025

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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

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科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 药理学 药理学是指药理学的学科.

背景情况:

  • 微RNA (miRs) 在细胞过程中起着至关重要的作用,并与许多疾病有关.
  • 针对 miRs 提出了开发新疗法的有吸引力的战略.
  • 开发精确的方法来调节miR活动对于有效治疗至关重要.

研究的目的:

  • 审查针对microRNAs的小分子药物发现的进展.
  • 突出小分子在调节与疾病相关的miR功能的潜力.
  • 讨论这些方法对 miR 向疗法和精准医学的变革性影响.

主要方法:

  • 能够选择性地准特定疾病相关的小分子的系统识别.
  • 在RNA研究和药物发现中利用多学科方法.
  • 开发可以抑制或降解miRs以调节其功能的化合物.

主要成果:

  • 在RNA研究的进步使特定的小分子的识别为miR准.
  • 小分子可以有效调节与疾病相关的miRs的活动.
  • 这些策略正在改变miR向治疗的格局.

结论:

  • 基于小分子的miRs调制提供了一个强大的治疗干预.
  • 这种方法意味着向各种疾病的精准医学迈出了一大步.
  • 讨论的多学科策略有可能彻底改变疾病治疗.