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Updated: Jun 26, 2025

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy
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单病毒分析:一种使用光显微镜可视化HIV-1粒子含量的方法.

Alice Duchon1, Ryan C Burdick2, Vinay K Pathak2

  • 1Viral Recombination Section, HIV Dynamics and Replication Program, National Cancer Institute, Frederick, MD, USA.

Methods in molecular biology (Clifton, N.J.)
|May 14, 2024
PubMed
概括

分析人类免疫缺陷病毒1型 (HIV-1) 病毒的含量对于理解病毒复制至关重要. 一种新的单核子分析方法使用光显微镜来确定单个HIV-1颗粒中的分子的存在和分布.

关键词:
光显微镜的光学显微镜.哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈艾滋病毒粒子成像技术RNA基因组的包装 RNA基因组的包装病毒组装组件 病毒组装组件

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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