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相关概念视频

Riboswitches01:56

Riboswitches

8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Types of RNA01:23

Types of RNA

63.5K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.5K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.4K
Termination of Translation01:44

Termination of Translation

25.3K
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
25.3K
Bacterial Transcription01:53

Bacterial Transcription

28.1K
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
28.1K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

29.4K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.4K

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相关实验视频

Updated: Jun 23, 2025

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

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科林环的修改揭示了B12-btuB Riboswitch相互作用的化学和空间要求.

Anastasia Musiari1, María Reichenbach1, Sofia Gallo1

  • 1Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland.

Chemistry (Weinheim an der Bergstrasse, Germany)
|June 26, 2024
PubMed
概括

这种btuB核突变器通过结合维生素B12 (AdoCbl) 来调节基因表达. 对维生素B12的修改

关键词:
用脚印进行测量.这是一个RNARNARNARNARNA.在Riboswitch中使用.维生素B12衍生物 维生素B12衍生物

更多相关视频

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
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Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

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An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

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相关实验视频

Last Updated: Jun 23, 2025

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

14.8K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

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An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

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科学领域:

  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.
  • 生物化学 生物化学

背景情况:

  • 这种btuB核突变器控制了BtuB外膜维生素B12载体的表达.
  • 感知维生素B12 (科巴胺) 的利博开关根据连接体大小被分为子类.
  • btuB 核糖转换器属于 I 类,特别结合腺甲胺 (AdoCbl).

研究的目的:

  • 调查维生素B12侧链b和e在btuB核突开关的识别和结构切换中的作用.
  • 确定这些侧链的化学修饰如何影响连接体-RNA结合亲和力和 рибо开关功能.

主要方法:

  • 利用直线探测来监测化学修饰对RNA结构和动态的影响.
  • 合成和测试化学修饰的维生素B12类似物和腺赛尔科巴胺 (AdoCbl).

主要成果:

  • 维生素B12侧链b的化学修饰显著影响了B12-RNA相互作用和结合亲和力.
  • 同样的修改在AdoCbl引入时具有较小的影响,这表明了更复杂的识别机制.
  • AdoCbl 的腺素部分在btuB рибо交换机识别中起着至关重要的作用,不仅仅是结合亲和力.

结论:

  • AdoCbl 的腺基对btuB рибо开关的特定识别和正确的结构切换至关重要.
  • 维生素B12与 рибо开关的侧链相互作用对于精确的基因调节至关重要.
  • 这些发现加深了对 ribowitch-ligand 相互作用和 B12 运输调节的理解.