Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Optimized Workflow from Gene to Product.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Rapid Two-Day Baculovirus Titering Using Flow Cytometry.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

The Paired-box protein PAX-3 regulates the choice between lateral and ventral epidermal cell fates in C. elegans.

Developmental biology·2016
Same author

Induction of functional Brm protein from Brm knockout mice.

Oncoscience·2015
Same author

Beyond Mutations: Additional Mechanisms and Implications of SWI/SNF Complex Inactivation.

Frontiers in oncology·2015
Same author

Possible evidence for re-regulation of HPA axis and brain reward systems over time in treatment in prescription opioid-dependent patients.

Journal of addiction medicine·2014
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
查看所有相关文章

相关实验视频

Updated: Jun 22, 2025

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

13.4K

为了高效的等离子体构造,TOPO克隆.

Maya Yovcheva1, Kenneth W Thompson2

  • 1Thermo Fisher Scientific, Frederick, MD, USA. Maya.Yovcheva@thermofisher.com.

Methods in molecular biology (Clifton, N.J.)
|July 1, 2024
PubMed
概括
此摘要是机器生成的。

托波克隆为Bac-to-Bac Baculovirus表达系统创建重组等离子体提供了一种快速的方法. 这种方法简化了昆虫细胞中蛋白质表达的基因克隆.

关键词:
百科病毒表达载体系统的表达载体系统感兴趣的基因感兴趣的基因这是TOPO克隆.

更多相关视频

Use of In Vivo Assembly for High-efficiency Plasmid Construction
06:25

Use of In Vivo Assembly for High-efficiency Plasmid Construction

Published on: February 7, 2025

420
Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

16.5K

相关实验视频

Last Updated: Jun 22, 2025

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

13.4K
Use of In Vivo Assembly for High-efficiency Plasmid Construction
06:25

Use of In Vivo Assembly for High-efficiency Plasmid Construction

Published on: February 7, 2025

420
Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

16.5K

科学领域:

  • 分子生物学分子生物学
  • 再组合DNA技术的使用.
  • 蛋白质表达系统 蛋白质表达系统

背景情况:

  • 背对背系统是昆虫细胞中高水平蛋白质表达的强大工具.
  • 有效地生成重组百科病毒对于成功的蛋白质生产至关重要.
  • 传统的克隆方法可能是耗时和劳动密集的.

研究的目的:

  • 描述一种简化方法,用于生成用于Bac-to-Bac系统的重组质粒.
  • 为了强调在这种情况下使用TOPO克隆的优点.

主要方法:

  • 使用TOPO克隆技术,快速将感兴趣的基因插入转移载体中.
  • 由此产生的重组等离子体随后用于转化为有能力的大肠杆菌.
  • 转变的大肠杆菌被选择和培养以获得所需的等离子体DNA.

主要成果:

  • 证明了TOPO克隆用于生成所需的重组等离子体的效率和速度.
  • 确认成功生成适合Bac-to-Bac系统后续步骤的等离子体.

结论:

  • TOPO克隆提供了一种高效且易于使用的方法,用于为Bac-to-Bac Baculovirus表达系统准备等离子体.
  • 这种方法显著加快了重组蛋白表达研究的工作流程.