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Updated: Jun 14, 2025

One-step Purification of Twin-Strep-tagged Proteins and Their Complexes on Strep-Tactin Resin Cross-linked With Bissulfosuccinimidyl Suberate BS3
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One-step Purification of Twin-Strep-tagged Proteins and Their Complexes on Strep-Tactin Resin Cross-linked With Bissulfosuccinimidyl Suberate BS3

Published on: April 20, 2014

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用 TEV 可切割的 His-Strep 标签对活性蛋白结合蛋白进行统一的净化方法

Daichi Nakajima1, Nozomi Takahashi2, Takanari Inoue3

  • 1Molecular Robotics Laboratory, Department of Robotics, Graduate School of Engineering, Tohoku University, Aoba 6-6-01 Aramaki Aoba-ku, Mechanical Eng. Research Bldg. 2 (A 03), Sendai, Miyagi, 980-8579, Japan.

MethodsX
|September 3, 2024
PubMed
概括

研究人员开发了一种统一的方法来净化细胞功能必不可少的关键活性蛋白结合蛋白. 这种高效的His-Strep-tag技术简化了调节actin细胞骨的蛋白质的制备.

关键词:
一种用于使用TEV可切割的His-Strep标签的动氨酸结合蛋白的统一净化方法.在ADF中,ADF是ADF.动氨酸细胞骨 动氨酸细胞骨封闭蛋白质是指封闭蛋白质的组成部分.科菲林是指一种可菲林.迷人的魅力 迷人他的标签是他的标签.个人资料 个人资料 个人资料链杆菌标签 - 链杆菌标签这是一个VASP系统.

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Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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