在早期玉米种子发育期间的染色质可访问性景观
Guang Ming Zheng1, Jia Wen Wu1, Jun Li1
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China.
The Plant journal : for cell and molecular biology
|March 24, 2025
概括
玉米种子中的染色素可访问性变化对于发展至关重要. 开放的染色体区域影响基因表达和特征相关的遗传变异,有助于玉米的育种进步.
科学领域:
- 植物生物学 植物生物学
- 基因组学就是基因组学.
- 发育生物学是发展生物学.
背景情况:
- 在可访问的染色体区域 (ACR) 中发现了Cis-regulatory元素 (CREs).
- 植物组织中的全基因组ACR已得到充分研究,但它们对玉米种子发育的影响尚不清楚.
研究的目的:
- 为了研究在早期玉米种子发育期间的染色质可访问性.
- 为了将ACR与基因表达相关联,并确定调节性转录因子 (TF).
- 探索ACRs在特征相关遗传变异中的作用及其在玉米育种方面的潜力.
主要方法:
- 用转化酶可访问的染色质序列测试 (ATAC-seq) 来绘制ACRs.
- 进行基因表达分析以与ACRs相关联.
- 在ACR中确定了TF约束足迹,以预测监管网络.
- 在NKD1基因中进行了包含特征相关SNP的ACR的编辑.
主要成果:
- 在玉米种子 (0-8 DAP) 中发现了37,952到59,887个高质量的ACR.
- 在促进剂-ACR和基因表达之间观察到正相关性.
- 确定了关键的TF家族和监管网络.
- 在ACR中发现DNA序列多样性的减少和特征相关SNP的丰富.
- 编辑NKD1 ACR与特征相关的SNP导致可观测的表型.
结论:
- 染色素的可获得性显著影响了玉米种子的发育.
- 开放的染色体区域对基因调节很重要,并具有与特征相关的变异.
- 了解ACR为改善玉米育种策略提供了潜力.
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