费里丁与MiniSOG融合,用于体外光动力疗法
Narathip Naradun1, Piyasiri Chueakwon1, Anyanee Kamkaew1
1School of Chemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
ACS applied bio materials
|April 22, 2025
概括
研究人员开发了HFn-miniSOG纳米粒子用于向光动力学疗法 (PDT). 这种融合蛋白针对过度表达人类费里受体 (TfR1) 的癌细胞,提供了一种新的癌症治疗方法.
科学领域:
- 生物技术是生物技术.
- 纳米医学是一种纳米医学.
- 癌症治疗 癌症治疗
背景情况:
- 与传统化疗和放射治疗相比,向性癌症疗法旨在最大限度地减少非向性影响.
- 人类费里受体 (TfR1) 在各种癌症中过度表达,使其成为药物输送的目标.
- 针对性治疗的现有药物封装方法耗时且具有局限性.
研究的目的:
- 设计一种新,简单的针对性癌症治疗方法.
- 为了创建一个融合蛋白,HFn-miniSOG,用于向光动力学疗法 (PDT).
- 开发自我组装的蛋白质纳米粒子,以有效治疗癌症.
主要方法:
- 工程人体费里 (HFn) 与迷你单片氧气发生器 (miniSOG) 融合.
- 标志着HFn-miniSOG自组装成纳米粒子 (平均尺寸为22.4±1.3纳米).
- 在蓝光激活时评估单个氧气生成,并在HepG2和HeLa细胞中评估光毒性.
主要成果:
- 在蓝光照射时,HFn-miniSOG纳米粒子有效地产生单个氧气 (ΦΔ = 0.30).
- 观察到有针对性的光毒性,HepG2细胞 (高TfR1) 在辐射后显示出63%的生存能力下降.
- 希拉细胞 (较低的TfR1) 仅表现出34%的活力降低,表明TfR1特异性向.
结论:
- HFn-miniSOG 作为一个有效的 ALL-IN-ONE 蛋白质纳米粒子,用于向的 PDT.
- 这种方法为开发向癌症治疗提供了一种简化和高效的方法.
- 这项研究强调了TfR1向蛋白质纳米颗粒在癌症治疗中的潜力.
相关概念视频
The Early Endosome: Endocytosis of Transferrin
3.2K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
3.2K
Transcytosis of IgG
2.7K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
2.7K
Tagging and Fusion Proteins
6.6K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.6K
Translocation of Proteins into the Mitochondria
3.0K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.0K
Protein Complexes with Interchangeable Parts
2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K
Vesicular Tubular Clusters
2.4K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
2.4K


