RiboParser/RiboShiny:一个用于全面分析和可视化Ribo-seq数据的集成平台
Shuchao Ren1, Yinan Li1, Zhipeng Zhou1
1National Key Laboratory of Agricultural Microbiology, College of Life Science, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Journal of genetics and genomics = Yi chuan xue bao
|April 23, 2025
概括
我们开发了RiboParser/RiboShiny,这是一个用户友好的框架,用于分析和可视化核糖体分析 (Ribo-seq) 数据. 这种工具提高了解释转化调节的准确性和效率,推进了基因表达研究.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 核糖体分析 (Ribo-seq) 彻底改变了体内转化调节的研究.
- 分析和可视化Ribo-seq数据存在重大挑战,需要改进的计算工具.
- 现有的分析管道往往缺乏全面性,准确性和用户友好性.
研究的目的:
- 开发一个强大的和用户友好的框架,RiboParser/RiboShiny,用于全面分析和可视化Ribo-seq数据.
- 为了提高P位检测在Ribo-seq分析中的准确性和稳定性,特别是对于具有挑战性的转录类型.
- 为各种有序基因组的生物提供一个普遍适用的平台.
主要方法:
- 基于核糖体结构和启动/停止模型的优化,用于P位检测.
- 开发RiboParser用于全面的Ribo-seq数据分析 (QC,基因水平,编码子水平,变体).
- 创建了RiboShiny,用于适应和用户友好的Ribo-seq数据可视化.
主要成果:
- RiboParser/RiboShiny框架显著提高了P位点检测的准确性和稳定性.
- 该平台提供全面的分析模块,包括质量控制和不同级别的基因分析.
- RiboShiny提供了直观的可视化工具,用于探索翻译景观.
结论:
- RiboParser/RiboShiny为Ribo-seq数据分析和可视化提供了一个强大的,集成的解决方案.
- 该框架提高了解释翻译法规的准确性和效率.
- 这种工具有可能显著推进基因表达和分子生物学方面的研究.
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