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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
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相关实验视频

Updated: Sep 15, 2025

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
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单核酸分辨率 经转录形状分析揭示了动态 m6 在牛植入前发展中的规则.

Rajan Iyyappan1, Yichi Niu2, Ming Hao1

  • 1Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32610, USA.

bioRxiv : the preprint server for biology
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概括

RNA N6 - 甲基氨酸 (m6A) 对于早期胚胎发育至关重要. 这项研究揭示了RPL12上一个特定的m6位点,该位点对牛胚胎发育至关重要,影响转化和基因表达.

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科学领域:

  • 表观遗传学和发育生物学
  • 哺乳动物胚胎发生
  • 基因组RNA的修改 基因组RNA的修改

背景情况:

  • RNA N6 - - 甲基氨酸 (m6A) 是基因表达的关键表观遗传调节器.
  • 在早期哺乳动物发育中的动态和功能作用尚未完全理解.
  • 之前的研究缺乏m6A在植入前发育过程中的单核酸分辨率.

研究的目的:

  • 以单核酸分辨率绘制牛卵细胞和植入前胚胎的全面地图.
  • 为了确定新的m6A修改对于早期胚胎发育至关重要.
  • 在关键的发育调节器中阐明特定站点m6A的功能意义.

主要方法:

  • 利用SAC-seq,一个单基分辨率,抗体独立的m6一个分析技术.
  • 产生了第一个全面的m6牛卵细胞和植入前胚胎的景观.
  • 研究了m6A修饰干扰对胚胎发育的功能影响.

主要成果:

  • 确定了RPL12转录中一个以前未经表征的m6A位点,该位点对胚胎发育至关重要.
  • 证明在这个部位失去m6A减少了蛋白质合成,并破坏了与翻译相关的基因表达.
  • 在m6A中断时观察到受损的阴茎基因组激活和胚胎细胞形成.
  • 表明野生类型的RPL12mRNA补充不能挽救发育停滞,突出显示了超出转录水平的m6A的作用.

结论:

  • 提供了有价值的单核酸分辨率 m6 哺乳动物胚胎发生的景观资源.
  • 揭示了一个关键机制,即特定于某个部位的m6A调节了早期胚胎的翻译和发育能力.
  • 表明m6在早期发育过程中,对精确控制蛋白质合成至关重要的一种修改.