发展时间分辨率发光度测量仪器用于生物传感和生物成像 - - 概述
Benjamin Sreenan1, Vala Kafil1, Donovan Wells1
1Department of Electrical and Biomedical Engineering, University of Nevada Reno, Reno, NV, 89577, USA.
Measurement : journal of the International Measurement Confederation
|July 21, 2025
概括
时间分辨率发光度测量 (TRLM) 使用长寿命探针进行高对比度生物感知. 本综述涵盖了TRLM仪器,它们的发展,以及移动应用的趋势.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 光学物理学 光学物理学
背景情况:
- 时间分辨率发光度测量 (TRLM) 使用具有衰变时间>100纳秒的探测器来增强生物传感和生物成像.
- 在TRLM中的脉冲延迟检测方法通过最大限度地减少光散射和自发光显著改善了信号与背景的比率.
研究的目的:
- 审查TRLM仪器对长寿命发光探针的最新发展和应用.
- 探索仪器开发趋势,以求在医疗中心 (POC) 和现场应用.
- 分析探头光学特性对TRLM仪器复杂性,成本,紧性和性能的影响.
主要方法:
- 对TRLM仪器和长寿命发光探针的现有文献的审查.
- 分析受探测器特性 (例如衰变时间,辐射频谱) 影响的仪器设计因素.
- 讨论用于TRLM的光源,探测器和电子设备的技术进步.
主要成果:
- 有广泛的长寿命探针 (有机染料,化物复合物,量子点,纳米粒子) 可用于TRLM.
- 已经开发了各种TRLM仪器,新的趋势是小型化和便携性.
- 探测器属性极大地影响仪器设计,成本和性能.
结论:
- TRLM为高对比度生物应用提供了一种强大的方法.
- 对于低成本,紧,高性能系统,需要进一步提高TRLM仪器仪表.
- 本次审查旨在促进对TRLM工具的更深入的理解,以便在生物传感和生物成像中得到更广泛的采用.
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