使用CRISPR增强相位敏感表面等离子体共振成像的原子核酸检测
Xiaoqi Dai1, Changle Meng1, Songfeng Huang1
1Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen 518060, China.
Analytical chemistry
|July 23, 2025
概括
一个新的集群定期间隔的短平行体重复 (CRISPR) 增强阶段查询表面等离子体共振成像 (CRISPR-PSPRi) 传感器提供了超敏感的实时核酸检测. 这种先进的诊断工具可以识别SARS-CoV-2和麻疹等病毒的特定DNA.
科学领域:
- 生物技术是生物技术.
- 生物感应是一种生物感应.
- 分子诊断学 分子诊断
背景情况:
- 传统的核酸检测方法缺乏临床诊断和病原体监测的灵敏度和动态范围.
- 挑战包括检测低度核酸和单核酸突变,阻碍实时,无放大应用.
研究的目的:
- 开发一种超敏感,实时,无放大,无标签的核酸检测传感器.
- 解决检测低度DNA和单核酸变异的灵敏度和动态范围的局限性.
主要方法:
- 开发了一种集群定期间隔的短平行体重复 (CRISPR) 增强相询表面等离子体共振成像 (CRISPR-PSPRi) 传感器.
- 利用CRISPR-Cas12a进行目标DNA识别和与ssDNA连接的金纳米粒子探针的转裂.
- 采用相延迟调制和波长扫描用于信号提取和动态范围扩展.
主要成果:
- 实现了高灵敏度 (1.436 × 10-6 RIU) 和广泛的动态范围 (0.0111 RIU).
- 成功检测到来自SARS-CoV-2 Omicron BA.2变种和病毒的特定DNA.
- 已证明可以检测到单核酸突变,低至1aM度.
结论:
- CRISPR-PSPRi传感器提供了一个实时,高通量和超敏感的核酸检测平台.
- 这项技术对推进临床诊断和病原体监测具有重大前景.
- 为无放大和无标签的核酸检测提供了可行的解决方案.
相关概念视频
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
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