Jove
Visualize
联系我们

相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

6.2K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
6.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Evaluating the efficiency of touch-spun scaffolds in producing dense cell cultures for tissue engineering applications.

Nanoscale·2026
Same author

Advances in Wearable Technology: MXene-Based Multifunctional and Biomedical Smart Textiles.

ACS omega·2026
Same author

Modeling Protein-Protein and Protein-Ligand Interactions by the ClusPro Team in CASP16.

Proteins·2025
Same author

Insulin-producing INS-1 cell cultures on biomimetic 3D scaffolds.

Journal of materials chemistry. B·2025
Same author

Strategies for fabricating aligned nano- and microfiber scaffolds: an overview for cell culture applications.

Nanoscale·2025
Same author

Hybrid Bismuth Halide with Rich Polymorphism and Second Harmonic Generation Response.

ACS materials letters·2025
Same journal

Ti/Sr Gradient Doping with SrTiO<sub>3</sub> Coating for Mitigating Strain and Oxygen Loss in Ni-Rich Cathode.

ACS applied materials & interfaces·2026
Same journal

Metallic Lead to Perfect Perovskite: A Bottom-Up Vapor-Assisted Colloidal Strategy for High-Performance Solar Cells.

ACS applied materials & interfaces·2026
Same journal

Two-Dimensional VSe<sub>2</sub>@Polypyrrole Heterostructure Enables Stable High-Rate Lithium-Sulfur Batteries.

ACS applied materials & interfaces·2026
Same journal

A Multifunctional Hydrogel Integrating Hemostatic, Antioxidant, and Antibacterial Properties for Infected and Diabetic Wound Regeneration.

ACS applied materials & interfaces·2026
Same journal

Tunable Interfacial to Filamentary Resistive Switching Mechanism in Room-Temperature-Grown Amorphous YBa<sub>2</sub>Cu<sub>3</sub>O<sub><i>x</i></sub> with Excess Cu Addition.

ACS applied materials & interfaces·2026
Same journal

Bioinspired Rhombic VO<sub>2</sub> Metasurface with Low Solar Absorptance for Self-adaptive All-Weather Building Thermal Management.

ACS applied materials & interfaces·2026
查看所有相关文章
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Sep 10, 2025

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

16.2K

基于非特定相互作用的无标签细胞分类的动态微结构热响应接口

Ronaldo Badenhorst1, Sergei V Makaev1, Mikhail Parker1

  • 1Nanostructured Materials Lab, University of Georgia, Athens, Georgia 30602, United States.

ACS applied materials & interfaces
|August 20, 2025
PubMed
概括
此摘要是机器生成的。

这项研究引入了使用热响应表面的新型无标签细胞分类. 这些表面通过控制粘附和脱落,实现高分离系数,从而实现有效的细胞分离.

关键词:
DPD模拟蒙特卡洛模拟细胞粘附没有标签的细胞分类在薄膜中分离相位可重新配置的微结构表面

更多相关视频

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
05:49

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

2.8K
Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications
05:09

Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications

Published on: February 2, 2024

1.5K

相关实验视频

Last Updated: Sep 10, 2025

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

16.2K
Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
05:49

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

2.8K
Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications
05:09

Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications

Published on: February 2, 2024

1.5K

科学领域:

  • 生物材料科学
  • 细胞生物学
  • 表面化学

背景情况:

  • 开发高效且无标签的细胞分类技术对于各种生物和医学应用至关重要.
  • 现有的方法通常需要细胞标记,这可能会影响细胞活力和功能.
  • 热敏聚合物为动态表面相互作用提供可调节的特性.

研究的目的:

  • 开发和描述用于无标签细胞分类的新型微结构热敏表面.
  • 研究这些表面的细胞粘附和脱落机制.
  • 通过控制表面特性和细胞相互作用来优化分类效率.

主要方法:

  • 微结构热敏表面的制造使用聚基酸 (PGMA) 和聚基酸 (PNIPAM-co-GMA) 通过相分离.
  • 微结构形成和温度诱导的膨胀/收缩的实验性表征.
  • 使用各种细胞系进行细胞分类实验 (RAW 264.7,NIH3T3/GFP,HaCaT).
  • 分散粒子动力学和蒙特卡洛模拟以建模细胞表面相互作用.

主要成果:

  • 使用可重新配置的热响应微结构表面证明成功的无标签细胞分类.
  • 通过调整相对于细胞粘合力的推向力来达到高分离因子 (> 50).
  • 鉴定了细胞分类的最佳条件,对细胞粘附性弱到中等有效.
  • 通过相分离条件和化时间证明了细胞粘附的可调性.

结论:

  • 微结构热敏表面为无标签的细胞分类提供了有效的平台.
  • 分类效率高度依赖于表面诱导力和细胞粘附力之间的平衡.
  • 这种方法在生物研究和诊断中为细胞分离提供了有前途的方法.