cloneXplorer:一个基于形微波阵列的高通量克隆发现平台
bioRxiv : the preprint server for biology
|February 6, 2026
概括
产生抗原特异性T细胞克隆现在通过cloneXplorer平台进行了简化. 这种活细胞分析系统有效地从周围血液中分离罕见的功能T细胞,用于免疫识别研究.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
背景情况:
- 产生抗原特异性T细胞种群对于理解免疫识别至关重要,但传统上涉及诸如限制稀释或克隆等繁的方法.
- 在免疫学研究中,需要高效和高通量方法来隔离和表征罕见的抗原特异性T细胞.
研究的目的:
- 开发和验证一种简化方法,直接从外周血液中生成抗原特异性T细胞克隆.
- 展示cloneXplorer活细胞分析和克隆分离平台的功能,用于识别和恢复功能定义的T细胞.
主要方法:
- 利用cloneXplorer平台,一个形微波阵列系统,用于活细胞分析和克隆隔离.
- 在单细胞共同培养中监测细胞增殖,细胞因子分泌 (干扰素玛) 和表面标记物.
- 采用光三明治测定用于免疫激活监测和四聚体染色用于特异性验证.
主要成果:
- 从细胞系和人类T细胞中有效生成单克隆细胞群.
- 在单细胞分辨率上成功识别了抗原特异性,使用工程化的Jurkat细胞和带有类库的抗原呈现细胞.
- 展示了基于干扰素玛分泌和CD8标志物表达的人类外周血液中抗原特异性T细胞的分离和扩张.
结论:
- 克隆Xplorer平台为生成抗原特异性T细胞克隆提供了强大而高效的解决方案.
- 这项技术在细胞系发育,免疫受体查和免疫反应验证方面具有广泛的应用.
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