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相关概念视频

Conformity01:20

Conformity

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Conformity is the change in a person’s behavior to go along with the group, even if that person does not agree with the group.
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It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
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Conformations of Butane02:20

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Unlike ethane and propane that have only two major conformations, butane has more than two conformers. The staggered form of butane in which the bulky methyl groups on the two carbons are placed on opposite sides, that is, at a dihedral angle of 180°, is the lowest energy, most stable form — called the anti conformer. This conformation is stabilized due to the absence of steric repulsion between the largely spaced out methyl groups. The other two staggered conformations are...
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Conformations of Cycloalkanes02:29

Conformations of Cycloalkanes

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Adolf von Baeyer attempted to explain the instabilities of small and large cycloalkane rings using the concept of angle strain — the strain caused by the deviation of bond angles from the ideal 109.5° tetrahedral value for sp3  hybridized carbons. However, while cyclopropane and cyclobutane are strained, as expected from their highly compressed bond angles, cyclopentane is more strained than predicted, and cyclohexane is virtually strain-free. Hence, Baeyer’s theory that...
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Cyclohexane does not exist in a planar form due to the high angle and torsional strain it would experience in the planar structure. Instead, it adopts non-planar chair and boat conformations.
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Conformations of Ethane and Propane02:18

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In an organic molecule, free rotation about the carbon-carbon single bond results in energetically different conformers of the molecule. Due to this rotation, called the internal rotation, ethane has two major conformations — staggered and eclipsed.
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用高带宽的纳米孔测量揭示隐藏的蛋白质构造.

Kyril Kavetsky1,2, Sabine Hong1, Chih-Yuan Lin1

  • 1Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.

Nano letters
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概括

先进的纳米孔测量揭示了6个不同的折叠状态的血红蛋白细胞染色体c. 这种技术提供了高分辨率的洞察力蛋白质动态和转位期间的形状变化.

关键词:
这是一个纳米孔.高带宽的高带宽带宽.低噪音放大器的放大器蛋白质折叠 蛋白质的折叠单个分子单个分子.超快速的蛋白质转位转位.

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科学领域:

  • 分子生物物理学 分子生物物理学
  • 纳米技术 纳米技术
  • 生物化学 生物化学

背景情况:

  • 纳米孔技术为分子结构探测提供了高空间和时间分辨率.
  • 了解蛋白质折叠动态在分子生物学和疾病研究中至关重要.

研究的目的:

  • 通过先进的纳米孔测量来研究血红蛋白细胞染色体c的多态折叠.
  • 通过纳米孔在转移过程中表征蛋白质状态和过渡.

主要方法:

  • 使用高信号对噪声,1-10 MHz带宽转移测量.
  • 采用了经过最佳设计的化纳米孔 (直径2.3-3.3纳米),并使用了变质剂 (Gdm-Cl).
  • 应用了~250kV/cm的电场用于蛋白质探测.

主要成果:

  • 根据使用贝叶斯信息标准分析的当前封锁水平确定了六种不同的蛋白质状态.
  • 在已识别的蛋白质状态之间计算过渡概率.
  • 确定了蛋白质从每个状态中退出孔隙的条件概率.

结论:

  • 该研究成功地使用纳米孔感应特征化了细胞染色体c的多态折叠.
  • 这些发现验证了纳米孔方法用于详细分析蛋白质结构动态.
  • 模拟事件证实了实验模型和数据解释.