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相关概念视频

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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In-situ Hybridization02:31

In-situ Hybridization

10.6K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
10.6K
Nuclear Overhauser Enhancement (NOE)01:06

Nuclear Overhauser Enhancement (NOE)

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Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...
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Osmoregulation in Fishes02:32

Osmoregulation in Fishes

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When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments?
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Network Covalent Solids02:18

Network Covalent Solids

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Network covalent solids contain a three-dimensional network of covalently bonded atoms as found in the crystal structures of nonmetals like diamond, graphite, silicon, and some covalent compounds, such as silicon dioxide (sand) and silicon carbide (carborundum, the abrasive on sandpaper). Many minerals have networks of covalent bonds.
To break or to melt a covalent network solid, covalent bonds must be broken. Because covalent bonds are relatively strong, covalent network solids are typically...
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Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Updated: Feb 14, 2026

Detection of Viral RNA by Fluorescence in situ Hybridization FISH
10:16

Detection of Viral RNA by Fluorescence in situ Hybridization FISH

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增强光的精度在现场混合 (FISH) 分析使用基于神经网络的核细分用于数字显微镜样本.

Annamaria Csizmadia1,2, Bela Molnar2,3, Marianna Dimitrova Kucarov4

  • 1Doctoral School of Pathological Sciences, Semmelweis University, H-1085 Budapest, Hungary.

Sensors (Basel, Switzerland)
|February 13, 2026
PubMed
概括
此摘要是机器生成的。

基于人工智能的3D核细分显著提高了在具有挑战性的淋巴瘤样本中光现场杂交 (FISH) 精度. 这种先进的方法在数字病理学工作流程中增强了核检测和基因偏差分类.

关键词:
细胞 摆设 摆设鱼类数量 鱼类数量核AIzer是一个核AIzer.星际距离 星际距离光在现场混合化光.毛囊性淋巴瘤是一种毛囊性淋巴瘤.核细分的核细分是指核细分.叠加的原子核重叠在一起.

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Fluorescence in situ hybridization FISH Protocol in Human Sperm
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Fluorescence in situ hybridization FISH Protocol in Human Sperm

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Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments
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相关实验视频

Last Updated: Feb 14, 2026

Detection of Viral RNA by Fluorescence in situ Hybridization FISH
10:16

Detection of Viral RNA by Fluorescence in situ Hybridization FISH

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Fluorescence in situ hybridization FISH Protocol in Human Sperm
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Fluorescence in situ hybridization FISH Protocol in Human Sperm

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Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments
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Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments

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科学领域:

  • 数字病理学数字病理学
  • 生物医学成像学 生物医学成像学
  • 基因组学就是基因组学.

背景情况:

  • 准确的核细分对于解释光现场杂交 (FISH) 结果至关重要.
  • 传统的二维自动化算法在淋巴瘤等样本中与密集或重叠的核斗争,失去重要的空间深度信息.
  • 这种限制影响了FISH诊断分析的可靠性.

研究的目的:

  • 评估基于人工智能的3D核细分是否可以提高FISH分析的准确性,可重现性和诊断可靠性.
  • 为了比较不同的AI算法 (NucleAIzer,StarDist,Cellpose) 和FISH中核细分的传统方法的性能.

主要方法:

  • 使用FISH.用BCL2基因重排序标记了形式素固定毛囊淋巴瘤部分.
  • 截面在多层Z堆中进行扫描,以捕获3D信息.
  • 人工智能算法 (NucleAIzer,StarDist,Cellpose) 和FISHQuant与人工眼睛控制对核细分精度进行了比较.

主要成果:

  • 2D细分方法和FISHQuant显示了密集核和低强度信号的局限性.
  • 人工智能驱动的3D细分改善了核分离和信号定位在焦点平面上.
  • 核AIzer和StarDist表现出卓越的精度,减少了差异 (VP/VS ≈0.96) 和强大的基因点相关性 (r > 0.82).

结论:

  • 不准确的核细分阻碍了FISH信号的自动评估.
  • 深度学习的3D细分模型,特别是NucleAIzer和StarDist,克服了2D方法的局限性.
  • 这些人工智能方法提高了核检测的一致性,从而改善了自动化数字病理学中基因异常的分类.