在NIR-I和NIR-II窗口中对DMDG-ICG进行光成像,使用单摄像头系统
Bonghwan Chon1, Mukesh P Yadav1, William Ghann2
1Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, 22 S. Greene St., Baltimore, MD 21201, USA.
International journal of molecular sciences
|February 27, 2026
概括
与纳米粒子成像的NIR-I相比,近红外II (NIR-II) 成像提供了更高的空间分辨率和对比度与噪声比率 (CNR). 一个单摄像头系统演示了NIR-II.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 光学工程是指光学工程.
- 纳米技术纳米技术
背景情况:
- 近红外 (NIR) 成像,包括NIR-I (800-1000 nm) 和NIR-II (1000-1700 nm),传统上使用单独的摄像头,阻碍了直接比较分析.
- 评估NIR-I和NIR-II性能需要能够同时在两个光谱窗口中获取数据的系统.
研究的目的:
- 调查单个摄像头系统是否可以在NIR-II中实现更高的空间分辨率和对比度与噪声比率 (CNR),而不是基于纳米粒子的成像NIR-I.
- 在NIR-I和NIR-II窗口中评估双模式,双Gd ICG (DMDG-ICG) 纳米颗粒的性能.
主要方法:
- 对DMDG-ICG纳米颗粒的吸收和光特性进行表征.
- 开发和利用一个定制的NIR成像系统,单一的InGaAs摄像头,用于同时采集NIR-I和NIR-II.
- 在体外和体内成像实验,包括测量全宽半最大 (FWHM) 和CNR在模仿组织的幽灵和小鼠模型中.
主要成果:
- 在NIR-I和NIR-II光谱区域中,DMDG-ICG纳米粒子表现出强烈的光.
- 观察到深度和组织层的扩散增加,在NIR-I中比NIR-II更大.
- 与NIR-I相比,NIR-II成像显示出明显较低的FWHM和更高的CNR,在内脂层深度高达10毫米 (p <0.05,n = 3).
- 在小鼠大腿动脉的体内成像显示,NIR-II的CNR比NIR-I更高 (p <0.05,n = 3).
结论:
- 一个单摄像头系统可方便直接比较,证实了NIR-II成像对NIR-I的优势.
- 在基于纳米粒子的应用中,NIR-II成像提供了增强的透度,优越的空间分辨率和改进的CNR.
- 这些发现支持NIR-II在先进的血管和分子成像方面的巨大潜力.
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