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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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相关实验视频

Updated: Mar 10, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

正角分散光谱单分子定位显微镜

Jun Lu¹, Lei Xu¹, Zhenyao Zhao¹

¹College of Biomedical Engineering Yiwu Research Institute Fudan University Shanghai China.

Nanophotonics (Berlin, Germany)

|March 9, 2026

概括

在单分子显微镜中,直角分散的sSMLM (ODsSMLM) 克服了光子预算的权衡. 这种新方法提高了先进的分子成像的空间定位和光谱表征.

科学领域:

生物物理学的生物物理.
光学显微镜的使用方法
频谱学是一种光谱学.

背景情况:

光谱单分子定位显微镜 (sSMLM) 能够同时获取空间和光谱数据.
在sSMLM的一个关键挑战是光子预算限制,迫使在本地化和光谱性能之间做出妥协.
现有的方法往往受到分散依赖的工件的影响,影响了定位精度.

研究的目的:

引入直角分散的sSMLM (ODsSMLM),以克服sSMLM中的光子预算困境.
为了使所有光子都可以用于定位和光谱表征.
为了实现同otropic横向定位精度和减少文物.

主要方法:

使用直角结构调节单分子排放光谱.
实施ODsSMLM用于同时获取空间和光谱数据.
用模拟数据和双色成像实验验证该方法的有效性.

主要成果:

ODsSMLM利用所有光子进行定位和光谱分析.
模拟数据显示了10纳米定位和1.1纳米光谱精度,使用3000光子预算.
微管和克拉特林的双色成像实现了27nm的同位素横向分辨率.

关键词:

正角分散的直角分散.单分子定位显微镜.这是光谱学.超高分辨率成像成像技术

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Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Published on: November 11, 2025

相关实验视频

Last Updated: Mar 10, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Published on: November 11, 2025

结论:

在sSMLM中,ODsSMLM有效地解决了局部化和光谱精度之间的权衡.
该方法提供了同otropic 横向定位,消除了依赖分散的工件.
ODsSMLM为多色成像和分子表征提供了显著的进步.