[Modified nephelometric method of determining the activity of extracellular staphylococcal plasmocoagulase]

The nephelometric method of the quantitative determination of plasmocoagulase was modified. The medium for growing staphylococci and the composition of the "substrate" were elaborated. The necessity of recalculation of the absolute coagulase activity into specific activity with the consideration to the microbial cell count in the suspension from which the supernatent containing the enzyme was obtained. The greatest plasmocoagulase activity was revealed in the 6- and the 24-hour cultures of Staph. aureus. The optimal time of incubation of the reactive medium (enzyme-substrate) was 4 hours.