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Related Experiment Videos

Systemic replacement therapy from genetically modified epidermal keratinocytes.

L B Taichman1

  • 1Department of Dermatology and Oral Biology and Pathology, State University of New York at Stony Brook, NY 11794-8702, USA.

Proceedings of the Association of American Physicians
|June 3, 1999
PubMed
Summary

Epidermal keratinocytes can deliver proteins to the bloodstream. This review examines the potential for gene transfer and systemic delivery using these skin cells.

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A partial catalog of proteins secreted by epidermal keratinocytes in culture.

The Journal of investigative dermatology·1999

Area of Science:

  • Dermatology
  • Molecular Biology
  • Genetics

Background:

  • Epidermal keratinocytes form the outermost layer of the skin.
  • These cells have potential applications in gene therapy and drug delivery.
  • Understanding protein secretion and systemic circulation is crucial for therapeutic development.

Purpose of the Study:

  • To review evidence supporting protein secretion by epidermal keratinocytes into the bloodstream.
  • To discuss factors influencing the quantity of protein delivered systemically by these cells.
  • To evaluate the potential of epidermal keratinocytes as a delivery vehicle for gene transfer.

Main Methods:

  • Literature review of existing studies on epidermal keratinocyte function.
  • Analysis of data on protein secretion and systemic absorption.

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  • Discussion of physiological and molecular factors affecting protein bioavailability.

    • Main Results:

    • Evidence confirms that proteins secreted by epidermal keratinocytes can enter the systemic circulation.
    • Factors such as protein size, stability, and epidermal barrier function influence delivery efficiency.
    • The quantity of protein delivered is dependent on secretion rate and absorption dynamics.

    Conclusions:

    • Epidermal keratinocytes represent a viable platform for systemic protein delivery.
    • Further research is needed to optimize gene transfer and therapeutic applications.
    • The skin's unique biology offers novel avenues for biotechnological advancements.