Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antisense inhibitory effect: a comparison between 3'-partial and full phosphorothioate antisense oligonucleotides.

U Galderisi1, G Di Bernardo, M A Melone

  • 1Institute of Pharmacology and Toxicoogy, C.R.I.S.C.E.B, Second University of Naples, Italy. galderisi@yahoo.com

Journal of Cellular Biochemistry
|June 25, 1999
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergism between different transport systems stimulates the uptake of neutral amino acids by isolated brain microvessels.

Amino acids·2013
Same author

Chronic renal failure, cachexia, and ghrelin.

International journal of peptides·2010
Same author

Expression profiles in surgically-induced carotid stenosis: a combined transcriptomic and proteomic investigation.

Journal of cellular and molecular medicine·2008
Same author

Pathophysiology of stem cells in restenosis.

Histology and histopathology·2007
Same author

Comparative evaluation of different DNA extraction procedures from food samples.

Biotechnology progress·2007
Same author

[Energy metabolism in cancer patients].

Minerva gastroenterologica e dietologica·2006

Partially phosphorothioated (PS) antisense oligonucleotides offer improved sequence specificity for inhibiting N-myc mRNA expression in neuroblastoma cells compared to full PS oligos. This makes them valuable tools for studying gene function over a wider concentration range.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Phosphorothioate (PS) antisense oligonucleotides are widely used to modulate cell functions.
  • Current PS oligonucleotides exhibit limited sequence specificity within a narrow concentration range.
  • N-myc gene expression is significantly elevated in human neuroblastoma cell lines.

Purpose of the Study:

  • To synthesize and evaluate partially phosphorothioated antisense oligomers targeting human N-myc mRNA.
  • To compare the sequence specificity and efficacy of partial PS oligos against full PS oligos.
  • To determine the utility of partial PS oligos for studying gene function.

Main Methods:

  • Synthesis of partially phosphorothioated antisense oligomers targeting human N-myc mRNA.

Related Experiment Videos

  • Treatment of human neuroblastoma cells with both partial and full PS antisense oligonucleotides.
  • Quantification of N-myc mRNA levels to assess inhibition.
  • Dose-response and time-course analysis of oligonucleotide efficacy.

    • Main Results:

    • Both partial and full PS oligos achieved maximum N-myc mRNA reduction at 6 hours.
    • Sustained inhibition for 20 hours required high concentrations for both types of oligos.
    • Partial PS oligos demonstrated dose-dependent and sequence-specific N-myc mRNA inhibition.
    • Full PS oligos showed disadvantages at higher concentrations, including reduced specificity.

    Conclusions:

    • Partial PS antisense oligonucleotides exhibit enhanced sequence specificity over a broader concentration range compared to full PS oligos.
    • Partial PS oligos are more suitable for studying gene function due to their improved specificity.
    • These findings suggest partial PS oligos are a valuable tool for gene function research in neuroblastoma.