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Determination of alternariol in tomato paste using solid phase extraction and high-performance liquid chromatography
C A Fente1, J Jaimez, B I Vázquez
1Dpto. de Química Analítica, Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, Spain.
The Analyst
|July 9, 1999
Summary
A new method accurately detects alternariol (AOH), a toxic metabolite from Alternaria molds, in tomato products. This research provides a reliable way to monitor AOH contamination in tomato paste, ensuring food safety.
Area of Science:
- Food Science
- Analytical Chemistry
- Mycology
Background:
- Alternaria species are common molds that contaminate agricultural products.
- Certain Alternaria species produce toxic metabolites, known as mycotoxins, which pose risks to human health.
- Tomato products are susceptible to Alternaria contamination, making them a potential dietary source of these toxins.
Purpose of the Study:
- To develop and validate a new analytical method for determining alternariol (AOH) in tomato paste.
- To establish a sensitive and precise method for mycotoxin analysis in food matrices.
Main Methods:
- Solid-phase extraction using cartridges for sample preparation.
- High-performance liquid chromatography (HPLC) with fluorescence detection.
- Reversed-phase column with isocratic elution for separation.
Main Results:
- The method demonstrated linearity in the 5.2–196 ppb range for AOH in tomato paste.
- High recovery rates for AOH were achieved (≥77.2%).
- A low detection limit of 1.93 ppb for AOH in real tomato paste samples was established.
- Excellent precision was observed, with repeatability (RSD = 2.98%) and reproducibility (RSD = 9.35%).
Conclusions:
- The developed HPLC method is suitable for the accurate determination of alternariol (AOH) in tomato paste.
- This method offers a sensitive, precise, and reliable tool for monitoring AOH contamination in food products.
- The findings contribute to ensuring the safety of tomato-based food products by enabling effective mycotoxin analysis.