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Determination of urinary orotic acid and uracil by capillary zone electrophoresis
C Salerno1, P D'Eufemia, M Celli
1Institute of Paediatrics and Department of Biochemical Sciences, University of Roma La Sapienza, Rome, Italy. ccrifo@axcasp.caspur.it
Journal of Chromatography. B, Biomedical Sciences and Applications
|November 26, 1999
Abstract:
We describe a simple method for measuring orotic acid and uracil concentration in urine by capillary zone electrophoresis in 20 mM Na-borate buffer, pH 9.2. The method was applied for studying a patient with HHH (hyperomithinemia, hyperammonemia and homocitrullinuria) syndrome. A high value of uracil excretion was found during periods of relatively low orotic acid excretion and normal ammonemia. The orotic acid level in urine was increased by increasing protein intake.