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Simultaneous determination of 5-hydroxyindoles and catecholamines by HPLC with fluorometric precolumn derivatization
1School of Pharmaceutical Sciences, Toho University, Chiba, Japan. iizuka@phar.toho-u.ac.jp
Advances in Experimental Medicine and Biology
|March 18, 2000
Abstract:
We have developed a highly sensitive and simple precolumn derivatization method for simultaneous determination of 5-hydroxyindoles (5HIs) and catecholamines (CAs) with 4-dimethylaminobenzylamine (DMBA). The method was successfully applied to the determination of 5HIs and CAs in plasma and urine.