In vitro selection of deoxyribozymes with DNA capping activity

In vitro selection was used to isolate a series of deoxyribozymes from a pool of random-sequence DNAs that catalyze an ATP-dependent self-capping reaction. Each deoxyribozyme catalyzes the transfer of the nucleoside and alpha-phosphate moieties of ATP to the phosphate group located at its 5' terminus, thereby creating a 5',5'-pyrophosphate cap. This same pyrophosphate cap structure is formed by T4 DNA ligase during the classical process of DNA ligation. These DNA capping enzymes representative of a collection of self-processing deoxyribozymes that can be used for the directed modification of DNA.