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Updated: Aug 15, 2026

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
Published on: March 22, 2012
Multiport-readout frame-transfer 5 megapixel CCD imaging system for TEM applications
1National Center for Microscopy and Imaging Research, Department of Neurosciences, School of Medicine, University of California, San Diego, La Jolla 92093-0608, USA. gfan@ucsd.edu
A new digital imaging system using a charge-coupled device (CCD) was developed for intermediate-high-voltage electron microscopy (IVEM). This system enables rapid image acquisition up to 400 keV, improving IVEM applications.
Area of Science:
- Electron Microscopy
- Digital Imaging Technology
- Solid-State Physics
Background:
- Intermediate-high-voltage electron microscopy (IVEM) requires advanced digital imaging systems for high-resolution analysis.
- Existing systems may have limitations in readout speed and sensitivity at higher electron energies.
Purpose of the Study:
- To develop and test a novel multiport-readout, frame-transfer charge-coupled device (CCD) digital imaging system for IVEM applications up to 400 keV.
- To evaluate the system's performance characteristics, including readout speed, sensitivity, and spatial resolution.
Main Methods:
- Development of a digital imaging system utilizing a back-thinned CCD with 2560 x 1960 pixels (24 µm x 24 µm pixel size).
- Implementation of a multiport readout architecture using four of eight on-chip ports in parallel, operating at 1- or 2-MHz pixel rates.
- Utilized frame-transfer readout as an electronic shutter for simultaneous integration and readout.
- Incorporated a thin film-based phosphor screen and lens relay for electron-to-digital signal conversion.
Main Results:
- Achieved a full CCD array readout time as short as 0.6 seconds.
- Demonstrated a conversion factor of 2.1 digital units per incident electron at 400 keV.
- Measured a modulation transfer function (MTF) of 14% at the Nyquist frequency, indicating good spatial resolution.
Conclusions:
- The developed multiport-readout, frame-transfer CCD system is suitable for IVEM applications up to 400 keV.
- The system offers rapid image acquisition and good performance characteristics for high-voltage electron microscopy.
- This technology advances digital imaging capabilities in high-energy electron microscopy.
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