Behavior of basic compounds in ion-exchange capillary electrochromatography with low-pH carrier electrolytes
Related Experiment Video
Updated: Aug 19, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Published on: March 2, 2012
1Separation Science Group, School of Chemistry, University of Tasmania, Hobart, Australia.
Abstract:
This work describes the separation of basic aromatic compounds by capillary electrochromatography employing acidic carrier electrolytes and bare silica as well as strong cation-exchange stationary phases. A mixed-mode separation mechanism was involved, comprising chromatographic interactions (adsorption, ion-exchange) as well as electrophoretic migration. The influence of ion-exchange on the retention/migration of the solutes could be manipulated according to procedures commonly employed in ion chromatography. These include variations of the eluting strength and/or the concentration of the competing ion present in the background electrolyte. Using this approach, separation times could be shortened and changes in selectivity could be achieved for a number of analytes.
More Related Videos
08:06Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
Published on: February 23, 2017
07:54Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
Published on: August 22, 2018
Related Concept Videos
Ion Exchange
Analyte Adsorption and Distribution
Ion-Exchange Chromatography
Electrophoresis: Overview
There...
Capillary Electrophoresis: Instrumentation
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...