[Method of mRNA differential display and its application in life science]

The changes of mRNA expression level control cell function. Many factors such as individual development, cell proliferation and differentiation, and even apoptosis, physiological stimuli, and drug treatment will change the level of gene expression. Comparisons of gene expression in different cell types provide the underlying information for analyzing the molecular and cellular mechanisms of the physiological processes. A method of mRNA differential display is developed recently, providing a powerful and useful tool, which can efficiently and rapidly isolate the genes that display a difference in transcription. These genes may play important role in cell function.