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Isolation and partial characterization of cDNA clone of human ceruloplasmin receptor
L K Sasina1, N V Tsymbalenko, N A Platonova
1Department of Molecular Genetics, Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg. ludmila@urs8.iem.ras.spb.ru
Bulletin of Experimental Biology and Medicine
|September 8, 2000
Abstract:
An individual clone, presumably carrying a 3 bp fragment of ceruloplasmin receptor cDNA was isolated from the expression library of human placenta cDNA using polyclonal specific antibodies to ceruloplasmin receptors. EcoR1-hydrolysate of isolated DNA was cloned in a pTZ19 bacterial vector and sequenced in the forward and reverse direction. The comparison of the revealed sequence with known sequences of human genome revealed its high similarity to ceruloplasmin cDNA.