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Detection of Legionella pneumophila using a real-time PCR hybridization assay

A L Ballard1, N K Fry, L Chan

  • 1Department of Microbiology and PHLS Laboratory, University Hospital, Nottingham NG7 2UH, United Kingdom.

Journal of Clinical Microbiology
|November 4, 2000
PubMed

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...