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Rat model of experimentally induced abacterial prostatitis
M D Lang1, J C Nickel, M E Olson
1Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada.
Background:
An experimental model in rats was developed to investigate the significance of mucosal integrity in abacterial prostatitis.
Methods:
Ethanol was instilled into the ventral prostates of male rats to reduce mucosal integrity; dinitrobenzenesulfonic acid (DNBS) was added as an irritant to induce inflammation. Controls received no treatment, ethanol only, DNBS only, or a suspension of bacteria. After various time points, rats were sacrificed, and their prostates were assayed for gross morphology, histological appearance, and cytokine levels.
Results:
Prostates subjected to ethanol plus DNBS showed significant inflammation, most notably after 12, 24, and 48 hr. Inflammation judged by gross and histological observations and interleukin-1beta levels correlated well at these times. Rats given only ethanol, DNBS, or no treatment, acting as negative controls, displayed little or no inflammation; rats given a bacterial suspension, acting as positive controls, showed inflammation consistent with past studies. Cytokine assays revealed raised interleukin-1beta levels in this model, while tumor necrosis factor-alpha remained at a basal level.
Conclusions:
The loss of an intact mucosal surface in the prostate resulted in inflammation caused by an irritant. Interleukin-1beta appears to play a role in this inflammation, while tumor necrosis factor-alpha does not.
Insights
Disrupting prostate mucosal integrity with an irritant induced inflammation in rats. Interleukin-1beta, not tumor necrosis factor-alpha, played a key role in this abacterial prostatitis model.
Area of Science:
- Urology
- Inflammation Research
- Animal Models
Background:
- Investigated the role of mucosal integrity in abacterial prostatitis using an experimental rat model.
- Abacterial prostatitis is a condition where inflammation of the prostate occurs without bacterial infection.
Purpose of the Study:
- To develop and validate an experimental model for studying abacterial prostatitis.
- To determine the significance of mucosal integrity in the development of prostate inflammation.
Main Methods:
- Created an experimental model in rats by instilling ethanol to compromise prostate mucosal integrity.
- Induced inflammation using dinitrobenzenesulfonic acid (DNBS) as an irritant, with controls receiving no treatment, ethanol only, DNBS only, or bacteria.
- Assessed prostates for gross morphology, histology, and cytokine levels (Interleukin-1beta, Tumor Necrosis Factor-alpha) at various time points.
Main Results:
- The combination of ethanol and DNBS caused significant prostate inflammation at 12, 24, and 48 hours.
- Inflammation observed through gross and histological analysis correlated well with elevated Interleukin-1beta levels.
- Elevated Interleukin-1beta was detected, while Tumor Necrosis Factor-alpha remained at basal levels in the inflamed prostates.
Conclusions:
- Loss of prostate mucosal integrity, when combined with an irritant, leads to inflammation.
- Interleukin-1beta is implicated in the inflammatory response in this abacterial prostatitis model.
- Tumor Necrosis Factor-alpha does not appear to play a significant role in this specific inflammatory model.