Related Experiment Video
Updated: Oct 10, 2026

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development
Published on: April 15, 2013
Combined liquid and gas chromatographic characterisation of polyglycerol fatty acid esters
B De Meulenaer1, G Van Royen, B Vanhoutte
1Department of Food Technology and Nutrition, Ghent University, Belgium.
Abstract:
In the present study a combined liquid and gas chromatographic technique is described for the analysis of polyglycerol fatty acid esters. Liquid chromatographic fractionation of samples resulted in pure standards of monoesters of di- and triglycerols and diesters of di- and triglycerols. Confirmation of their identity was achieved by LC-MS analysis. Moreover, a chromatographic identification of the mono- and diesters of cyclic diglycerol was proposed. From the isolation of pure esters and their gas chromatographic analysis, it was revealed that co-elution of several compounds occurred. Thus it was shown that prefractionation of the sample using a simplified liquid chromatographic separation, was necessary in order to characterise the esters correctly. In combination with some other chemical analyses, a complete profile of the chemical composition of polyglycerol fatty acid esters can be obtained.
Related Concept Videos
Gas Chromatography: Introduction
In GC, a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column.
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Chromatographic Methods: Classification
Chromatographic techniques are typically named by...

