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Thin-layer chromatographic analysis of fludarabine and formycin A in human plasma
1Department of Medicinal Chemistry, Medical Academy, 6 Chodźki Str., 20-093 Lublin, Poland.
Abstract:
Fludarabine and formycin A, extracted from plasma, were separated by TLC method on silica gel and aluminium oxide by horizontal development, using suitable mobile phases. The substances were visualized by UV irradiation. After extraction from plasma it was possible to detect 100 ng of fludarabine and 200 ng of formycin A. These results suggested that the TLC system is useful for the initial detection and identification of these drugs in emergencies.
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