Isolation and partial identification of immunoglobulin from T cells

Using fowl anti mouse (Fab)2 antibody, a protein has been collected from culture fluid and cell lysates of thymoma cells and stimulated thymocytes. This protein did show an immunoglobulin arc on immunoelectrophoresis against abovementioned antibody and against rabbit anti mouse Ig serum. No precipitation lines have been produced by mono-specific anti-mouse gamma, mu and alpha sera. On reduced PAGE heavy and light chains were present. Mobility of the heavy chain was between mu and gamma chains and its apparent molecular weight was calculated at appr. 65,000 daltons. The light chain mobility corresponded to mouse serum kappa chains.