Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stacking in capillary zone electrophoresis.

Z K Shihabi1

  • 1Pathology Department, Wake Forest University, Baptist Medical Center, Winston-Salem, NC 27157, USA. zshihabi@wfubmc.edu

Journal of Chromatography. A
|February 24, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis and classification of serum cryoglobulins.

Methods in molecular medicine·2011
Same author

Myoglobin analysis.

Methods in molecular medicine·2011
Same author

Enzyme analysis : cathepsin d as an example.

Methods in molecular medicine·2011
Same author

Serum lamotrigine analysis.

Methods in molecular medicine·2011
Same author

Acetonitrile stacking : serum phenobarbital as an example.

Methods in molecular medicine·2011
Same author

Comparison of glycated albumin and hemoglobin A(1c) levels in diabetic subjects on hemodialysis.

Kidney international·2008
Same journal

Impurity profiling of lipid-conjugated oligonucleotides using reversed-phase with and without ion-pair reagents and hydrophilic interaction liquid chromatography.

Journal of chromatography. A·2026
Same journal

Preparation of magnetic zwitterionic covalent organic frameworks for rapid simultaneous extraction of hydrophilic and hydrophobic organophosphates from environmental waters coupled with UHPLC-MS/MS determination.

Journal of chromatography. A·2026
Same journal

Analysis of organic and inorganic acids in biomass pyrolysis process samples by ion chromatography-mass spectrometry.

Journal of chromatography. A·2026
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
Same journal

Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification.

Journal of chromatography. A·2026
Same journal

Ion chromatography: Current strengths, key limitations, and future trends.

Journal of chromatography. A·2026
See all related articles

Sample stacking in capillary zone electrophoresis (CZE) significantly enhances detection limits. This technique uses discontinuous buffers to concentrate analytes, improving sensitivity for practical applications.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrophoresis (CE) has limited detection limits due to short light paths.
  • High-Performance Liquid Chromatography (HPLC) offers superior detection limits for many applications.
  • Concentration detection limits in CE are often insufficient for practical sample analysis.

Purpose of the Study:

  • To review methods for sample concentration (stacking) in capillary zone electrophoresis (CZE).
  • To discuss the role of discontinuous buffers in analyte stacking.
  • To highlight the improvement in CE detection limits achieved through stacking.

Main Methods:

  • Review of various electrophoretic maneuvers for sample stacking in CZE.
  • Explanation of discontinuous buffer systems and their function in analyte velocity manipulation.

Related Experiment Videos

  • Discussion of different mechanisms for on-capillary concentration.

    • Main Results:

    • Stacking methods can achieve concentration factors of 5-30 fold.
    • Improved detection limits in CZE approach those of HPLC.
    • Discontinuous buffers are crucial for effective band sharpening and analyte stacking.

    Conclusions:

    • Sample stacking significantly enhances CE detection limits, making it comparable to HPLC.
    • Discontinuous buffers are key to achieving effective analyte concentration in CZE.
    • Further development of stacking methods is needed for broader practical applications of CZE.