Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasma sample preparation by ultrafiltration for FTIR analysis.

A Aslani1, R D Hansen, B A Cooper

  • 1Department of Nuclear Medicine, Royal North Shore Hospital, St Leonards, NSW Australia. aaslani@bigfoot.com

Australasian Physical & Engineering Sciences in Medicine
|February 24, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of the COD: N ratio on mainstream deammonification in an integrated fixed-film activated sludge sequencing batch reactor.

Chemosphere·2020
Same author

Theoretical optimal cricothyroidotomy incision length in female subjects, following identification of the cricothyroid membrane by digital palpation.

International journal of obstetric anesthesia·2018
Same author

Impact of a Pilot Videogame-Based Physical Activity Program on Walking Speed in Adults with Schizophrenia.

Community mental health journal·2017
Same author

Smart micro/nanoparticles in stimulus-responsive drug/gene delivery systems.

Chemical Society reviews·2016
Same author

The symptom phenotype of oncology outpatients remains relatively stable from prior to through 1 week following chemotherapy.

European journal of cancer care·2016
Same author

The effect of weight reduction on ultrasonographic findings of nonalcoholic Fatty liver.

Middle East journal of digestive diseases·2014
Same journal

A comparison between EPSON V700 and EPSON V800 scanners for film dosimetry.

Australasian physical & engineering sciences in medicine·2020
Same journal

Nanodosimetric understanding to the dependence of the relationship between dose-averaged lineal energy on nanoscale and LET on ion species.

Australasian physical & engineering sciences in medicine·2020
Same journal

Schizophrenia diagnosis using innovative EEG feature-level fusion schemes.

Australasian physical & engineering sciences in medicine·2020
Same journal

Force decoding using local field potentials in primary motor cortex: PLS or Kalman filter regression?

Australasian physical & engineering sciences in medicine·2020
Same journal

EPSM 2019, Engineering and Physical Sciences in Medicine : 28-30 October 2019, Perth, Australia.

Australasian physical & engineering sciences in medicine·2020
Same journal

New name: Physical and Engineering Sciences in Medicine.

Australasian physical & engineering sciences in medicine·2020
See all related articles

Ultrafiltration (UF) offers an efficient and accurate alternative to vacuum sublimation (VS) for preparing plasma samples. This new method improves the determination of total body water (TBW) using Fourier Transform Infrared (FTIR) analysis.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Total body water (TBW) is a critical physiological parameter that can be altered by disease.
  • Current gold standard methods for TBW measurement, such as isotope dilution, are costly, time-consuming, and require specialized expertise.
  • Plasma sample preparation is a significant bottleneck in existing TBW determination techniques.

Purpose of the Study:

  • To evaluate ultrafiltration (UF) as an efficient and accurate alternative to vacuum sublimation (VS) for plasma sample preparation.
  • To assess the suitability of UF for Fourier Transform Infrared (FTIR) determination of TBW.
  • To investigate the impact of urea concentration on the UF method.

Main Methods:

  • Deuterium Oxide (D2O) concentrations were prepared in human plasma.

Related Experiment Videos

  • Plasma samples were subjected to both ultrafiltration (UF) and vacuum sublimation (VS) for sample preparation.
  • Fourier Transform Infrared (FTIR) analysis was performed on prepared samples and D2O standards in distilled water.
  • Paired t-tests were used for statistical comparison of absorbance values and urea effects.

    • Main Results:

    • UF plasma samples showed no significant difference compared to D2O standards (p=0.9).
    • VS plasma samples showed a significant difference compared to D2O standards (p=0.003).
    • The UF technique demonstrated higher sample throughput and efficiency.
    • A marginal effect of high urea concentration on UF was observed at higher D2O levels (p=0.04 at 1.6 g/L).

    Conclusions:

    • Ultrafiltration (UF) is an accurate and more efficient method for plasma sample preparation in FTIR-based TBW determination.
    • UF overcomes the limitations of traditional vacuum sublimation (VS) methods.
    • This novel UF approach offers a practical advancement for measuring total body water.