Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA electrochemical biosensors.

M Mascini1, I Palchetti, G Marrazza

  • 1Dipartimento di Sanità Pubblica, Epidemiologia e Chimica Analitica Ambientale, Università di Firenze, Via G. Capponi, 9, 50121, Firenze, Italy. mascini@unifi.it

Fresenius' Journal of Analytical Chemistry
|February 24, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Crocins pattern in saffron detected by UHPLC-MS/MS as marker of quality, process and traceability.

Food chemistry·2018
Same author

Electrochemical aptasensors for contaminants detection in food and environment: Recent advances.

Bioelectrochemistry (Amsterdam, Netherlands)·2017
Same author

Real-time kinetic binding studies at attomolar concentrations in solution phase using a single-stage opto-biosensing platform based upon infrared surface plasmons.

Optics express·2017
Same author

Acetamiprid multidetection by disposable electrochemical DNA aptasensor.

Talanta·2016
Same author

Measurement of volatile organic compounds (VOCs) in libraries and archives in Florence (Italy).

The Science of the total environment·2016
Same author

Alkaline-Phosphatase-Based Nanostructure Assemblies for Electrochemical Detection of microRNAs.

Journal of nanoscience and nanotechnology·2015

Disposable electrochemical DNA biosensors offer rapid screening for toxic compounds and detection of DNA hybridization. These sensors utilize DNA interactions to identify pollutants and specific genetic sequences in environmental and biological samples.

Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Nucleic Acid Analysis

Background:

  • Disposable electrochemical DNA biosensors are emerging tools for analyzing biological and environmental samples.
  • These sensors leverage the specific interactions of DNA with target molecules or sequences.
  • Previous applications focused on detecting low-molecular-weight compounds and hybridization events.

Purpose of the Study:

  • To review the applications of disposable electrochemical DNA biosensors.
  • To highlight their use in determining compounds that interact with nucleic acids.
  • To demonstrate their capability in detecting DNA hybridization reactions.

Main Methods:

  • Utilizing surface-linked DNA on electrodes to detect target compounds via their effect on guanine oxidation signals.

Related Experiment Videos

  • Employing chronopotentiometric analysis for signal measurement.
  • Immobilizing synthetic oligonucleotides on graphite screen-printed electrodes for specific DNA sequence detection.
  • Using daunomycin as an indicator for hybridization events.

    • Main Results:

    • DNA biosensors successfully detected intercalating compounds like daunomycin, polychlorinated biphenyls (PCBs), aflatoxin B1, and aromatic amines.
    • The sensors demonstrated applicability in analyzing river and wastewater samples.
    • Detection of specific DNA sequences through hybridization was achieved, with potential application to apolipoprotein E (ApoE) after PCR amplification.

    Conclusions:

    • Disposable electrochemical DNA biosensors are versatile for detecting both small molecules and specific DNA sequences.
    • These sensors show promise for rapid screening of environmental pollutants and contaminants in food.
    • Further development could extend their use to diverse environmental and clinical diagnostics.