[Analysis of spleen polyribosomal RNA during primary and secondary immune responses]

Synthesis of polyribosomal RNA was studied in the cells of mouse spleen, primarily and secondarily immunized with rat erythrocytes. In development of the immune response there was observed an increase in the number of polyribosomes which could occur first of all on account of intensification of their synthesis and secondly on account of their stabilization with the new informative RNA. Polyribosomal RNA labeled with P32 was analyzed in polyacrylamide gel. Electrophoresis of polyribosome preparations treated with a 2% SDS was conducted. It was found that in the secondary immune response in the 18S--4S there were revealed two principal activity peaks; other iRNA, which participated in the cellular growth of the organ realizing the antibody production, were synthesized during the primary immune response.