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Related Experiment Videos

Formaldehyde production by Tris buffer in peptide formulations at elevated temperature.

Y Song1, R L Schowen, R T Borchardt

  • 1Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS 66047, USA.

Journal of Pharmaceutical Sciences
|September 6, 2001
PubMed
Summary
This summary is machine-generated.

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Tris buffer degrades at high temperatures, releasing formaldehyde. This formaldehyde can then react with peptides, impacting formulation stability and requiring careful consideration for peptide and protein storage.

Area of Science:

  • Biochemistry
  • Pharmaceutical Sciences
  • Chemical Engineering

Background:

  • Tris buffer is a common buffering agent in biological and pharmaceutical formulations.
  • Peptide and protein formulations require stable excipients for maintaining drug integrity.
  • Elevated temperatures can accelerate degradation pathways in complex formulations.

Purpose of the Study:

  • To investigate the stability of Tris buffer in a peptide formulation at elevated temperatures.
  • To identify potential degradation products of Tris buffer under stress conditions.
  • To assess the impact of buffer degradation on peptide integrity.

Main Methods:

  • Peptide formulation containing Tris buffer was stored at 70°C.
  • High-performance liquid chromatography (HPLC) was used to analyze buffer and peptide integrity.

Related Experiment Videos

  • Mass spectrometry was employed to identify degradation products.
  • Chemical assays were performed to detect formaldehyde.

    • Main Results:

    • Tris buffer demonstrated significant degradation at 70°C.
    • Formaldehyde was identified as a primary degradation product.
    • Formaldehyde was observed to react with tyrosine residues on the peptide.
    • Degradation led to changes in peptide characteristics.

    Conclusions:

    • Tris buffer is unstable in peptide formulations at elevated temperatures.
    • Formaldehyde release from Tris buffer poses a risk of chemical modification to peptides.
    • Peptide/protein formulation scientists must consider Tris buffer instability at high temperatures.