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Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
Published on: October 1, 2015
[cDNA cloning and sequence analysis of human lactoferrin]
1Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|November 13, 2001
Summary
Researchers sequenced human lactoferrin (hLF) cDNA from Chinese mammary tissue, finding 99% homology with existing sequences but identifying two key genetic variations. These variations result in a specific amino acid change in the hLF protein.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Context:
- Human lactoferrin (hLF) plays crucial roles in immunity and iron transport.
- Understanding genetic variations in hLF is important for its therapeutic applications.
- Mammary tissue is a primary source for hLF production.
Purpose:
- To amplify and sequence human lactoferrin (hLF) cDNA from normal human mammary tissue.
- To analyze the genetic sequence of hLF and compare it with existing databases.
- To identify any significant variations in the hLF cDNA sequence.
Summary:
- Human lactoferrin (hLF) cDNA was successfully amplified from normal human mammary tissue using RT-PCR.
- The determined hLF cDNA sequence (2136 bp) exhibited 99% homology with five other registered hLF sequences.
- Two critical base substitutions (G-->C at nucleotide 1740 and T-->C at nucleotide 1756) were identified, leading to an amino acid change (Glu-->Asp at residue 580).
Impact:
- Identifies specific genetic variations in human lactoferrin.
- Provides valuable data for research into hLF function and potential therapeutic uses.
- Contributes to the understanding of human lactoferrin genetic diversity.
