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Changes in surface charge of low-density lipoproteins during oxidative modification
R K Aidyraliev1, O A Azizova, M M Mirrakhimov
1National Center of Cardiology and Therapy, Kirghiz Ministry of Health, Bishkek. cardio@elcat.kg
Bulletin of Experimental Biology and Medicine
|November 20, 2001
Summary
Oxidative modification of low-density lipoproteins (LDL) increases their negative surface charge over time. This charge alteration, measured by fluorescence probes, may contribute to the atherogenic activity of oxidized LDL.
Area of Science:
- Biochemistry
- Cardiovascular Research
- Lipid Metabolism
Background:
- Low-density lipoproteins (LDL) are key carriers of cholesterol.
- Oxidative modification of LDL is implicated in atherosclerosis.
- Changes in LDL surface charge may influence its biological activity.
Purpose of the Study:
- To investigate the effect of oxidative modification on the surface charge of LDL.
- To quantify the changes in LDL surface charge over time during autooxidation.
- To explore the role of lipid peroxidation in LDL surface charge alterations.
Main Methods:
- Autooxidation of LDL at 37°C for varying durations (6h, 24h).
- Incubation of LDL with EDTA to inhibit lipid peroxidation.
- Quantification of LDL oxidative modification using fluorescence probes.
- Measurement of LDL surface charge.
Main Results:
- LDL negative surface charge increased progressively with oxidation time.
- A 2-fold increase in surface charge was observed after 24h of oxidation.
- Short-term oxidation (6h) showed minimal changes in surface charge.
- EDTA treatment prevented changes in surface charge, indicating lipid peroxidation's role.
Conclusions:
- Oxidative modification significantly alters the negative surface charge of LDL.
- The extent of surface charge modification correlates with the duration of LDL autooxidation.
- These charge alterations are likely mediated by lipid peroxidation and may contribute to atherogenesis.