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Related Experiment Videos

Nomofungin: a new microfilament disrupting agent.

A S Ratnayake1, W Y Yoshida, S L Mooberry

  • 1Department of Chemistry, University of Hawaii at Manoa, 2545 McCarthy Mall, Honolulu, HI 96822-2275, USA.

The Journal of Organic Chemistry
|December 26, 2001
PubMed
Summary

A novel alkaloid, nomofungin, was discovered from an endophytic fungus. This compound disrupts microfilaments in mammalian cells and shows moderate cytotoxicity, with an unprecedented ring system.

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Area of Science:

  • Natural Product Chemistry
  • Mycology
  • Cell Biology

Background:

  • Endophytic fungi residing in medicinal plants are a rich source of novel bioactive compounds.
  • Ficus species are known to harbor diverse endophytic microorganisms with potential pharmacological activities.

Purpose of the Study:

  • To isolate and characterize a new alkaloid from an endophytic fungus found on Ficus microcarpa.
  • To elucidate the chemical structure and absolute stereochemistry of the novel compound.
  • To evaluate the biological activity, including cytotoxicity and cellular effects, of the isolated alkaloid.

Main Methods:

  • Fermentation of an endophytic fungus isolated from Ficus microcarpa bark.
  • Structure elucidation using spectroscopic methods (e.g., NMR, Mass Spectrometry).

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  • Determination of absolute stereochemistry via the exciton chirality method.
  • Cytotoxicity assays against LoVo and KB cell lines.
  • Microfilament disruption assays in cultured mammalian cells.

    • Main Results:

    • Isolation and characterization of a new alkaloid named nomofungin.
    • Determination of the unprecedented ring system structure and absolute stereochemistry of nomofungin.
    • Nomofungin demonstrated moderate cytotoxicity with minimum inhibitory concentrations (MICs) of 2 µg/mL against LoVo cells and 4.5 µg/mL against KB cells.
    • The compound was found to disrupt microfilaments in cultured mammalian cells.

    Conclusions:

    • Nomofungin is a novel alkaloid with a unique chemical structure isolated from an endophytic fungus.
    • The compound exhibits moderate cytotoxic effects and disrupts the cellular microfilament network.
    • This discovery highlights the potential of endophytic fungi from Ficus species as a source for new bioactive natural products.