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Modulatory effects of autologous plasma on functional activity of human immunocompetent cells
O P Ryabchikov1, V I Kirillov, A N Tsygin
1Institute of Human Morphology, Russian Academy of Medical Sciences.
Insights
Autologous plasma treatment modulated immune cell activity in children with kidney diseases. This treatment impacted lymphocyte blast transformation and natural killer cell function, varying with disease stage.
Area of Science:
- Immunology
- Pediatric Nephrology
Background:
- Pyelonephritis and glomerulonephritis are significant pediatric kidney diseases.
- Immune cell function, including lymphocyte blast transformation and natural killer cell activity, plays a role in kidney disease pathogenesis.
- Understanding immune responses in pediatric kidney diseases is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the effects of autologous plasma on blast transformation of lymphocytes and natural killer cell activity in children with pyelonephritis and glomerulonephritis.
- To assess whether autologous plasma treatment modulates immune responses in pediatric kidney disease patients.
- To determine the relationship between disease stage and the modulation of immune cell activity by autologous plasma.
Main Methods:
- Studied blast transformation of peripheral blood lymphocytes stimulated with phytohemagglutinin and concanavalin A.
- Assessed natural killer cell activity in children with pyelonephritis before and after treatment with 50% autologous plasma.
- Correlated immune cell activity changes with the stage of pyelonephritis and glomerulonephritis.
Main Results:
- Autologous plasma significantly modulated blast transformation of lymphocytes in children with pyelonephritis and glomerulonephritis.
- Natural killer cell activity in children with pyelonephritis was altered by autologous plasma treatment.
- The extent of modulation of lymphocyte and natural killer cell activity by autologous plasma was dependent on the stage of the kidney disease.
Conclusions:
- Autologous plasma can modulate key immune cell functions in pediatric kidney diseases.
- The therapeutic potential of autologous plasma in pyelonephritis and glomerulonephritis may be linked to its immunomodulatory effects.
- Disease stage is a critical factor influencing the response to autologous plasma treatment in pediatric nephrology.
Abstract:
Blast transformation of peripheral blood lymphocytes stimulated with phytohemagglutinin and concanavalin A was studied in children with pyelonephritis and glomerulonephritis. Activity of natural killer cells from children with pyelonephritis was estimated before and after treatment with 50% autologous plasma. The autologous plasma modulated blast transformation of lymphocytes and activity of natural killer cells, which depended on the stage of diseases.