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Updated: Oct 2, 2026

The MPLEx Protocol for Multi-omic Analyses of Soil Samples
Published on: May 30, 2018
Solid-phase microextraction as a sample preparation strategy for the analysis of seleno amino acids by gas
Anne P Vonderheide1, Maria Montes-Bayon, Joseph A Caruso
1Department of Chemistry, University of Cincinnati, Ohio 45221-0172, USA.
Abstract:
Solid-phase microextraction (SPME) is used as a sample preparation strategy for gas chromatographic (GC) analysis of the seleno amino acids, selenomethionine (SeMet), selenoethionine (SeEt) and selenocystine (SeCys). Acylation of the amino group and esterification of the carboxylic group in these compounds was performed with isobutylchloroformate to increase volatility. The amino acid derivatives were then extracted by silica fibers with polydimethylsiloxane (PDMS) coatings prepared by the sol-gel process. Investigations of extraction time, acid and salt addition, and polymer length (for the sol-gel process) were conducted with the goal of procedural optimization. Initial characterizations were conducted using gas chromatography with flame ionization detection (GC-FID). Inductively coupled plasma mass spectrometric detection was employed for final selenium detection. Sub-ppb detection limits were obtained for all analytes although relative standard deviations were higher than those typically obtained in solid-phase microextraction.
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