Related Experiment Videos
Chromium toxic effect monitoring using ozonation method
T Poznyak1, J M Puga, E Kiseleva
1Superior School of Chemical Engineering, National Polytechnic Institute of Mexico, Mexico D.F., Mexico. tpoznyak@hotmail.com
International Journal of Toxicology
|June 11, 2002
Summary
Hexavalent chromium exposure causes lipid peroxidation (LPO) in plasma, erythrocytes, and semen. Ozonation offers a fast, inexpensive method to measure this chromium toxicity using double bond indexes.
Area of Science:
- Environmental toxicology
- Biochemistry
- Analytical chemistry
Background:
- Hexavalent chromium is a toxic environmental pollutant.
- Chromium exposure can induce oxidative stress and lipid peroxidation (LPO).
- Accurate and rapid methods for detecting chromium toxicity are needed.
Purpose of the Study:
- To evaluate the in vitro toxicity of hexavalent chromium on lipids in plasma, erythrocytes, and semen.
- To propose and validate the ozonation technique for measuring chromium-induced LPO.
- To establish double bond indexes (DB-index and DB(cell)-index) as indicators of chromium's effect on lipids.
Main Methods:
- In vitro exposure of plasma, erythrocytes, and semen lipids to hexavalent chromium.
- Application of the ozonation technique for lipid peroxidation measurement.
- Calculation of double bond indexes (DB-index and DB(cell)-index).
Main Results:
- Ozonation successfully detected the effect of hexavalent chromium on lipid peroxidation.
- DB-index and DB(cell)-index values correlated with chromium exposure.
- The method demonstrated quantitative, rapid, and inexpensive measurement capabilities.
Conclusions:
- Ozonation is a viable technique for detecting chromium-induced LPO in biological samples.
- DB-index and DB(cell)-index determination serves as a reliable measure of chromium toxicity.
- The proposed ozonation method is a suitable modern approach for in vitro chromium toxic effect monitoring.