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Updated: Aug 27, 2026

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
Published on: August 26, 2016
Determination of phosphine in biogas and sludge at ppt-levels with gas chromatography-thermionic specific detection
Joris Roels1, Herman Van Langenhove, Willy Verstraete
1Laboratory of Microbial Ecology and Technology, Faculty of Applied and Biological Sciences, Ghent University, Belgium.
Abstract:
A gas chromatographic (GC) system to measure free phosphine in biogas and matrix bound phosphine in manure and sludge is presented. The system consists of a sample preconcentration trap filled with glass beads, connected with a capillary GC equipped with a thermionic specific detector. With a trap temperature as low as -155 degrees C, a sampling flow of 20 ml/min and a typical total sample volume of 100 ml, free phosphine concentrations in the low ng/m3 range and matrix bound phosphine in the low ng/kg dry matter range, can be accurately and reproducibly determined.
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