Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interference in immunoassay is an underestimated problem.

A A A Ismail1, P L Walker, M L Cawood

  • 1Department of Clinical Chemistry, Pinderfields General Hospital, Wakefield WF1 4DG, UK. dr.ismail@panp-tr.northy.nhs.uk

Annals of Clinical Biochemistry
|July 16, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reinforcement learning driven adaptive active frequency drift for fast and reliable islanding detection.

Scientific reports·2026
Same author

Use of eGFR instead of creatinine in exclusion criteria for derivation of calcium adjustment equation.

Annals of clinical biochemistry·2021
Same author

Evaluation of the MP Rapid 2019-NCOV IgM/IgG combo POCT test vs. an established platform-based method.

Annals of clinical biochemistry·2021
Same author

Albumin and calcium reference interval using healthy individuals and a data-mining approach.

Annals of clinical biochemistry·2020
Same author

The impact of the analytical performance specifications of calcium and albumin on adjusted calcium.

Annals of clinical biochemistry·2020
Same author

The effect of different analytical platforms and methods on the performance of population-specific adjusted calcium equation.

Annals of clinical biochemistry·2020
Same journal

Routine laboratory triage for NT-proBNP elevation may reflect cardiorenal frailty as much as heart failure specificity.

Annals of clinical biochemistry·2026
Same journal

From interpretable NT-proBNP prediction to equitable laboratory stewardship.

Annals of clinical biochemistry·2026
Same journal

Serum Sclerostin Levels in Children with Osteogenesis Imperfecta.

Annals of clinical biochemistry·2026
Same journal

Population-Specific HOMA-IR Reference Ranges in Iraq: Defining Insulin Sensitivity in a Middle Eastern Cohort.

Annals of clinical biochemistry·2026
Same journal

Optimizing Laboratory Quality with Sigma Metrics: Application of CLIA 2024 Total Allowable Error Guidelines.

Annals of clinical biochemistry·2026
Same journal

Suspected thermal amplitude of cryoglobulin likely more clinically significant than size detected: A case report.

Annals of clinical biochemistry·2026
See all related articles

Patient antibodies can interfere with immunoassay accuracy, a persistent issue. Understanding antibody-antigen interactions is crucial for clinical laboratories to manage this unpredictable diagnostic challenge.

Area of Science:

  • Immunology
  • Clinical Chemistry
  • Analytical Chemistry

Background:

  • Patient antibodies in serum are a known cause of immunoassay interference.
  • This interference is often observed with new analytes and persists despite manufacturer efforts.
  • Case reports highlight the problem, but a deeper understanding of immunoanalytical principles is needed.

Purpose of the Study:

  • To explain the immunoanalytical basis of antibody interference in assays.
  • To raise awareness among clinical laboratories about this unpredictable issue.
  • To discuss the prevalence, influencing factors, and future trends of antibody interference.

Main Methods:

  • Review of antibody usage as reagents in immunoassays.
  • Analysis of factors governing antibody-antigen binding.

Related Experiment Videos

  • Examination of the immune response mechanisms.
  • Discussion of prevalence studies and their limitations.

    • Main Results:

    • Interference prevalence varies widely (0.05% to >2%), being analyte- and assay-specific.
    • The problem is intrinsic and unpredictable, despite manufacturer compensation attempts.
    • Increased use of monoclonal antibodies may worsen future prevalence and incidence.

    Conclusions:

    • Clinical laboratories must remain vigilant for antibody-mediated immunoassay interference.
    • Immunoassays remain essential diagnostic tools, necessitating awareness of potential interferences.
    • Understanding antibody-antigen interactions is key to managing this diagnostic hurdle.