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Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination
Kazuki Nakajima1, Yasuo Oda, Mitsuhiro Kinoshita
1Faculty of Pharmaceutical Sciences, Kinki University, Higashi-osaka, Japan.
The Analyst
|August 14, 2002
Abstract:
We examined the formation of complexes between terbium ion (Tb3+) and carbohydrates labeled with aminobenzene compounds. Of the examined compounds, carbohydrates labeled with 4-aminosalicylic acid showed intense fluorescence with Tb3+ in the presence of cetylpyridinium chloride at pH 6.0. Calibration curves for maltose derivative showed good linearity between 5 pmol and at least 600 pmol, with good reproducibility. We applied the proposed technique to binding studies between manno-oligosaccharides and Concanavalin A.