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Cholinesterase measurements with an automated kit
Georgino H Oliveira1, John D Henderson, Barry W Wilson
1Department of Animal Science, University of California, Davis, California 95616, USA.
American Journal of Industrial Medicine
|September 5, 2002
Summary
The Test-mate kit shows inconsistent acetylcholinesterase (AChE) activity measurements across temperatures and between kits. Field use is not recommended without an enzyme activity standard for validation.
Area of Science:
- Biochemistry
- Clinical Diagnostics
Background:
- The Test-mate kit measures blood acetylcholinesterase (AChE) and hemoglobin.
- Previous models exhibited temperature-dependent inconsistencies.
- This study evaluates the current ChE 400 and older OP models.
Purpose of the Study:
- To assess the accuracy and reliability of the current Test-mate ChE 400 model.
- To compare its performance with previous OP models.
- To evaluate Test-mate kit performance across a range of temperatures (10-37°C).
Main Methods:
- Acetylcholinesterase (AChE) activity was measured using the Ellman assay.
- Three Test-mate kits and a 96-well microplate reader were utilized.
- Fetal bovine serum served as the source of AChE.
Main Results:
- Normalized AChE activities decreased below 20°C (ChE model) and 25°C (OP models).
- Inter-kit variability in AChE activity measurements was observed (1.03–1.49 µmol/min/mL).
- Test-mate kits showed higher percent errors compared to the microplate reader.
Conclusions:
- The current Test-mate model (ChE 400) is not recommended for fieldwork.
- Previous field measurements using Test-mate kits may be unreliable.
- Parallel use of an enzyme activity standard is recommended for Test-mate kit usage.