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Rapid determination of sanitizer concentration using impedance-based methods.
Gianna M Duran1, Douglas L Marshall
1Department of Food Science and Technology, Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, Mississippi State 39762-9805, USA.
Journal of Food Protection
|September 18, 2002
Summary
Rapid test kits and Bactometer impedimetric methods accurately measure sanitizer concentrations. The Bactometer offers a fast, reliable alternative for analyzing chlorine, iodophor, and quaternary ammonium compounds.
Area of Science:
- Microbiology
- Analytical Chemistry
- Food Safety
Background:
- Sanitizer concentration verification is crucial for effective disinfection.
- Rapid and accurate methods are needed for routine quality control.
Purpose of the Study:
- To evaluate the suitability of three Bactometer impedimetric test codes (impedance, conductance, capacitance) for assaying chlorine, iodophor, and quaternary ammonium solutions.
- To compare the performance of these impedimetric methods against standard rapid test kits.
Main Methods:
- Assaying sanitizer solutions using rapid test kits and three Bactometer impedimetric test codes.
- Determining the optimal test code for each sanitizer type (sodium hypochlorite, iodophor, quaternary ammonium).
- Performing linear regression analysis to compare Bactometer results with test kit results.
Main Results:
- Impedance, conductance, and capacitance test codes were found most suitable for sodium hypochlorite, iodophor, and quaternary ammonium solutions, respectively.
- Strong linear correlations (R2 values 0.87-0.99) were observed between Bactometer and test kit results across various concentrations.
- The impedimetric methods provided rapid (6 min) and highly certain (P < 0.0002) measurements.
Conclusions:
- Bactometer impedimetric methods are simple, rapid, and accurate alternatives for quantifying common sanitizer concentrations.
- These methods can significantly save time in quality control settings, especially for multiple sample analyses.