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Collagen resorption by fibroblasts in human gingiva
R M Frank1, G Cimasoni, A Tsamouranis
1Groupe de Recherches I.N.S.E.R.M. U 157, Faculté de Chirurgie dentaire, Université Louis-Pasteur, Strasbourg, France.
Journal De Biologie Buccale
|December 1, 1977
Summary
Fibroblasts in the lamina propria form intracellular vacuoles containing collagen fibrils during gingival reattachment after flap surgery. These vacuoles exhibit varying matrix densities, impacting collagen visibility.
Area of Science:
- Cell Biology
- Connective Tissue Research
- Oral Surgery
Background:
- Flap surgery and gingival reattachment involve significant tissue remodeling.
- Fibroblasts play a crucial role in connective tissue repair and matrix formation.
- Intracellular processes within fibroblasts during healing are not fully understood.
Purpose of the Study:
- To investigate the ultrastructural changes in fibroblasts during gingival reattachment following flap surgery.
- To characterize the contents and morphology of intracellular vacuoles observed in lamina propria fibroblasts.
Main Methods:
- Light and electron microscopy were employed to examine fibroblast ultrastructure.
- Tissue samples from patients undergoing flap surgery and subsequent gingival reattachment were analyzed.
- Detailed observation of intracellular vacuoles and their contents (collagen fibrils) was performed.
Main Results:
- Numerous intracellular vacuoles were identified in fibroblasts of the lamina propria.
- These vacuoles contained one or more collagen fibrils.
- The matrix within the vacuoles varied, appearing either electron-lucent or electron-dense, which affected the visibility of fibril outlines and cross-striations.
Conclusions:
- Intracellular vacuoles containing collagen fibrils are a feature of fibroblasts during gingival healing after flap surgery.
- The observed vacuoles may represent a mechanism for collagen processing or transport within fibroblasts.
- Further research is needed to elucidate the precise function of these vacuoles in tissue remodeling and reattachment.